Fig. 1

Comparison of a biomass productivity (g L⁻¹ day⁻¹) and b lipid productivity (mg L⁻¹ day⁻¹) of S. quadricauda LWG002611 with other eight isolated oleaginous microalgae grown in autotrophic cultivation condition using BBM and mixotrophic cultivation condition using TAP media where acetate is supplemented as external carbon source in uniform cultivation condition at a temperature of 27 °C ± 0.5 °C, a photoperiod of 14:10 h light/dark cycle and fluorescent illumination of 3000 lux. Productivity was estimated in logarithmic phase of growth. c Comparison of biomass productivity (g L⁻¹ day⁻¹) and lipid productivity (mg L⁻¹ day⁻¹) of S. quadricauda LWG002611 with earlier reports of microalgae grown in different carbon sources i.e. mixotrophic (mixo) growth in acetate and autotrophic (auto) growth in CO₂ and air. Bar (i) indicate our estimation, bar (ii, vi, vii, ix) indicate the estimation by Rodolfi et al. [9], bar (iii, iv) by Mandal and Mallick [26], bar (v) by Yoo et al. [27], bar (viii) by Chiu et al. [28] and bar (x, xi) by Liang et al. [29]. d Comparison of S. quadricauda LWG002611 large-scale production rates (TOE ha⁻¹ year⁻¹) with other promising oleaginous microalgae as well as with other biofuel feedstocks. Bar (i) indicate the average global productivity of microalgal lipid [11], bar (ii) indicate our estimations for S. quadricauda LWG002611, extrapolated from 1 L cultures cultivated in TAP medium at a temperature of 27 °C ± 0.5 °C, a photoperiod of 14:10 h light/dark cycle and fluorescent illumination of 3000 lux; bar (iii) indicate extrapolated estimations of lipid productivity of Nannochloropsis gaditana in nitrogen deficient condition [18], bar (iv) indicate the lipid productivity of Chlorella sp. [18], bar (v), (vi), (vii) and (viii) represent the oil productivity of Jatropha [18], Palm [1], Sunflower [1] and Rapeseed [1] respectively. e The extracted lipid (mg) from biomass (g) of S. quadricauda LWG002611 was refluxed for 5 h at 50 °C in the presence of methanol and 2% sulphuric acid for transesterification. After removal of impurities the FAME mix (mg mg⁻¹ of lipid) was dissolved in hexane and estimated for the percentage of Fatty acid methyl ester (FAME) (weight/weight) by Gas-Chromatography (Thermo Fisher Scientific) and quantified against a standard FAME mix (Supelco, USA) (values are from three separate experiments and error bars show the standard deviation)