Table 3 Strains and plasmids in this study
Strains | Characteristic | Source |
|---|---|---|
B. subtilis 168 | Wild type | ATCC |
B. licheniformis | ||
WX-02 | Wild-type strain, CCTCC M208065 | CCTCC |
WX02-glpK | WX-02 derivative, over-expression of glpK | This study |
WX02-glpP | WX-02 derivative, over-expression of glpP | This study |
WX02∆tglpFK | WX-02 derivative, defective in tglpFK | This study |
WX02-zwf | WX-02 derivative, over-expression of zwf | This study |
WX02-tkt1 | WX-02 derivative, over-expression of tkt1 | This study |
WX02-tkt2 | WX-02 derivative, over-expression of tkt2 | This study |
WX02-rpe | WX-02 derivative, over-expression of rpe | This study |
WX02-gndA | WX-02 derivative, over-expression of gndA | This study |
WX02-pgi | WX-02 derivative, over-expression of pgi | This study |
WX02-fba | WX-02 derivative, over-expression of fba | This study |
WX02-glpX | WX-02 derivative, over-expression of glpX | This study |
BC2 | WX02-glpK derivative, over-expression of glpX | This study |
BC3 | BC2 derivative, over-expression of zwf | This study |
BC4 | BC3 derivative, over-expression of tkt1 | This study |
E. coli DH5a | F−Φ80d/lacZΔM15, Δ(lacZYA-argF)U169, recA1, endA1, hsdR17 (r −K , m +K ), phoA, supE44, λ−, thi-1, gyrA96, relA1 | Laboratory stock |
Plasmids | ||
T2(2)-ori | Bacillus knockout vector; Kan r | Laboratory stock |
T2-GglpK | T2(ori)-glpK(A+B+glpK); to over-express glpK | This study |
T2-GglpP | T2(ori)-glpP(A+B+glpP); to over-express glpP | This study |
T2-∆tglpFK | T2(ori)-tglpFK(A+B); to knock out tglpFK | This study |
T2-Gzwf | T2(ori)-zwf(A+B+glpK); to over-express glpK | This study |
T2-Gtkt1 | T2(ori)-tkt1(A+B+tkt1); to over-express tkt1 | This study |
T2-Gtkt2 | T2(ori)-tkt2 (A+B+tkt2); to over-express tkt2 | This study |
T2-Grpe | T2(ori)-rpe(A+B+rpe); to over-express rpe | This study |
T2-GgndA | T2(ori)-gndA(A+B+gndA); to over-express gndA | This study |
T2-Gpgi | T2(ori)-pgi(A+B+pgi); to over-express pgi | This study |
T2-GglpX | T2(ori)-glpX(A+B+glpX); to over-express glpX | This study |
T2-Gfba | T2(ori)-fba(A+B+fba); to over-express fba | This study |