Fig. 4

Heat map analysis showing changes in the contents of metabolites during Camelina seed development. The data represent the metabolite content ratios in Camelina transgenic DGAT1, GPD1, and D + G lines relative to WT at 10–16 DAF, 18–26 DAF, and 28–36 DAF. WT data was used as controls in pairwise comparisons. Metabolites showed a ratio of < 1.00 and significant difference (P ≤ 0.05) are highlighted in orange, metabolites narrowly missed statistical cutoff for significance 0.05 < P < 0.10 and metabolite ratio of < 1.00 are highlighted in light orange, metabolites showed a ratio of ≥ 1.00 and significant difference (P ≤ 0.05) are highlighted in blue, metabolites narrowly missed statistical cutoff for significance 0.05 < P < 0.10 and metabolite ratio of ≥ 1.00 are highlighted in light blue, and non-colored text and cell means values are not significantly different for that comparison. Values are representative of four biological replicates of developing seeds bulked from at least 8 plants for each time point. The genotypes used are WT, wildtype, DGAT1, AtDGAT1 overexpressor, GPD1, ScGPD1 overexpressor, and D + G, GPD1 + DGAT1 overexpressor. Developing seeds were harvested at 10–16 DAF (WT-16, GPD1–16, DGAT1–16, D + G-16), 18–26 DAF (WT-26, GPD1–26, DGAT1–26, D + G-26), 28–36 DAF (WT-36, GPD1–36, DGAT1–36, D + G-36)