Fig. 2

Detection of delivered fluorescently labeled Cas9 proteins within strain KJ cells. Fluorescently labeled Cas9 proteins were mixed with strain KJ cells in an electroporation cuvette; the cells were not electroporated (a, b and c) or electroporated using the “optimum” conditions described in the text (d, e, and f). Then, cells were analyzed by FACS without washing (a and d) or after three (b and e) or five washings (c and f) with PBS buffer to eliminate the fluorescently labeled Cas9 protein adsorbed on cell surface. The numbers within each panel are percentages of gated events (namely, detection of highly fluorescent cells)