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Table 1 Efficiency of genome editing using Cas9-gRNA RNP

From: Cas9-guide RNA ribonucleoprotein-induced genome editing in the industrial green alga Coccomyxa sp. strain KJ

  Expt 1 Expt 2-1 Expt 2-2 Expt 2-3 Expt 2-4
Electric field strength of poring pulse
(V cm−1)
7500 7500 5000
Dark incubation period (day) 7 2 7 2 7
Total number of colonies examined 10,760 11,724 11,892 12,306 11,880
Number of knockout lines obtained 1 1 2 0 0
Gene-editing efficiency (%) 0.01 0.01 0