Cas9-guide RNA ribonucleoprotein-induced genome editing in the industrial green alga Coccomyxa sp. strain KJ

Table 1 Efficiency of genome editing using Cas9-gRNA RNP

	Expt 1	Expt 2-1	Expt 2-2	Expt 2-3	Expt 2-4
Electric field strength of poring pulse (V cm⁻¹)	7500	7500		5000
Dark incubation period (day)	7	2	7	2	7
Total number of colonies examined	10,760	11,724	11,892	12,306	11,880
Number of knockout lines obtained	1	1	2	0	0
Gene-editing efficiency (%)	0.01	0.01		0

ISSN: 2731-3654