Fig. 1From: PLDα1-knockdown soybean seeds display higher unsaturated glycerolipid contents and seed vigor in high temperature and humidity environmentsGeneration of soybean PLDα1 knockdown lines. Soybean PLDα1KD transgenic plants were generated by stable transformation of soybean cultivar Jack with a soybean PLDα1 RNA interference (RNAi) construct. Transgenic soybean plants were used for analysis. a Construction of soybean PLDα1KD. 1151bp one in forward and the other in reverse and separated by a DNA fragment of Pdk-I gene, is driven by a seed specific promoter for soybean β-conglycinin gene encoding a major seed storage protein in seed, and terminated by a Phaseo gene terminator. b Immunoblotting screening PLDα1 in seeds of transgenic soybean plants 1020 (PLDα1KD1). 1–6 indicate individual plants from transgenic line or wild-type Jack (control). c Immunoblotting detection PLDα1 in seeds of another transgenic soybean line 1048 (PLDα1KD2). 1–3 indicate the individual plants from each transgenic line or wild-type Jack (control). d Immunoblotting of PLDα1 using proteins extracted from leaves and seeds from two PLDα1KD lines1. e PLDα1 activity in two PLDα1KD lines 1 and wild-type Jack line1 (control) developing seedsBack to article page