Fig. 6

Phospholipid profiles in PLDα1KD and wild-type developing seeds. a Profiles of phospholipids in mature seeds of KD (PLDα1KD) and wild-type Jack (WT) by ESI-MS/MS. All seeds collected from soybean plants grown under normal conditions. b Total lipid contents of major lipids and the molecular species (total acyl chains: double bonds). All plants were grown under high temperature and humidity. All plants were grown under high temperature and humidity. The values are the mean ± S.D. (n = 4 or 5). WT-2: wild-type Jack at stage 2; KD1-2: knockdown line 1 at stage 2; KD2-2: knockdown line 2 at stage 2; The rest can be explained in the same manner. All * and **denote significance at P < 0.05 and P < 0.01, respectively, compared with wild-type Jack (WT) based on Student’s t test