Table 1 List of identified 1D-resolved protein spots from control and ASW responsible in Scenedesmus sp. IITRIND2
Band ID | Identification in | Protein name | Function categories | Theoretical/actual MW (KD) | Mascot score | Coverage (%) | Subcellular location | Fold change |
|---|---|---|---|---|---|---|---|---|
1 | A0A1D1ZZ74_AUXPR | Uncharacterized protein | Unknown | 200/207.9 | 57 | 14 | Unknown | 3.5 (↓) |
2 | C1E424_MICC | Uncharacterized protein (fragment) | Helicase activity (predicted) | 150/111.5 | 88 | 14 | Unknown | – |
3 | K8F2R1_9CHLO | Malic enzyme
 | Lipid biosynthesis | 76/78 | 69 | 15 | Mitochondria | 1.4 (↑) |
A0A087SEW0_AUXPR | Alpha-1,4 glucan phosphorylase | Starch synthesis | 76/100.2 | 77 | 19 | Chloroplast | ||
4 | C1DY39_MICCC | Peptidylprolyl isomerase | Protein degradation | 70/63.3 | 76 | 13 | Cytoplasm | 4.3 (↑) |
A0A0D2LKL19_CHLO | Glucose-6-phosphate isomerase | Glycolysis | 70/64.59 | 144 | 27 | Cytoplasm | ||
5 | Q6PYY4_OSTTA | Starch synthase | Starch synthesis | 60/58.7 | 74 | 16 | Chloroplast | 1.78 (↓) |
E1ZTI5_CHLVA | Glucose-1-phosphate adenylyltransferase | Starch synthesis | 60/55.6 | 75 | 19 | Chloroplast | ||
6 | A0A172C330_9CHLO | Ribulose bisphosphate carboxylase large chain (fragment) | Calvin cycle | 52/36.59 | 69 | 30 | Chloroplast | 1.34 (↓) |
7 | C0SKA4_9CHLO | Tubulin beta chain (fragment)

 | Flagellar motion/cytoskeleton microtubule | 40/41.6 | 87 | 22 | Cytoplasm | 1.42 (↓) |
8 | A8JHU0_CHLRE | Malate dehydrogenase
 | TCA cycle | 31/38.8 | 59 | 16 | Mitochondria | – |
A0A059LS29_9CHLO | Catalase
 | Antioxidant | 31/57.37 | 96 | 13 | Cytoplasm | ||
A0A0D2M0Q2_9CHLO | 3-ketoacyl-CoA synthase
 | Lipid biosynthesis | 31/33.9 | 71 | 21 | Chloroplast | ||
9 | A0A097KLR8_9CHLO | Mg-protoporphyrin IX chelatase | Chlorophyll synthesis | 27/39.6 | 77 | 36 | Chloroplast | 1.5 (↓) |
10 | A0A150H154_GONPE | Uncharacterized protein | Unknown | 26/31.4 | 71 | 21 | Unknown | 1.2 (↓) |
11 | A0A1D1ZTJ7_AUXPR | Uncharacterized protein | Unknown | 25/20.87 | 68 | 20 | Unknown | 1.1 (↓) |
12 | D8TLP0_VOLCA | Glutathione peroxidase | Detoxification of H2O2 | 24/22.27 | 67 | 11 | Cytoplasm | – |
13 | A0A061S0Z1_9CHLO | Phosphodiesterase | Hydrolase activity | 20/48.2 | 52 | 14 | Cytoplasm | 2.3 (↓) |
14 | A8J7H6_CHLRE | Thioredoxin-like protein | Antioxidant | 19/27.6 | 67 | 29 | Cytoplasm | – |
A0A06IRYCR_9CHLO | Pyrroline-5-carboxylate reductase | Proline synthesis | 19/28.3 | 78 | 29 | Cytoplasm | ||
A0A1C9ZQC69_CHLO | Alfin-like protein | Salt tolerance | 19/20.1 | 52 | 17 | Cytoplasm | ||
15 | C1KR5_MICCC | 50S ribosomal protein L14 | Protein synthesis | 15/13.40 | 87 | 40 | Chloroplast | 1.1 (↑) |
Q946N2_9CHLO | Ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit (fragment) | Calvin cycle | 15/21.8 | 71 | 45 | Chloroplast | ||
16 | A0A06IQQ83_9CHLO | Nadh:ubiquinone oxidoreductase 13 kDa subunit | Oxidative phosphorylation | 12/16.3 | 52 | 20 | Chloroplast | 1.2 (↓) |
17 | A0A061QN19_9CHLO | Putative salt tolerance-like protein (fragment) | Salt tolerance | 10/7.9 | 54 | 48 | Unknown | – |