Table 2 Fermentation parameters in YPX medium at 30 and 40 °C in oxygen-deprived conditions
Strain | Temp (°C) | Xylosei (g/L) | Xylosef (g/L) | Ethanolf (g/L) | Xylitolf (g/L) | Glycerolf (g/L) | Yet/xyl (g/g) | Yxyol/xyl (g/g) | DCW (g/L) |
|---|---|---|---|---|---|---|---|---|---|
PE-2∆GRE3 | 30 | ||||||||
XR/XDHa | 51.7 ± 0.1 | 3.11 ± 0.13 | 10.9 ± 0.0 | 24.4 ± 0.3 | 1.44 ± 0.00 | 0.225 ± 0.002 | 0.503 ± 0.007 | 5.78 ± 0.53 | |
XR/XDH + XIb | 51.7 ± 0.1 | 3.82 ± 0.20 | 10.2 ± 0.1 | 25.3 ± 0.2 | 0.921 ± 0.007 | 0.214 ± 0.002 | 0.527 ± 0.003 | 5.43 ± 0.34 | |
XIc | 51.7 ± 0.1 | 0.00 ± 0.00 | 22.4 ± 0.1 | 1.91 ± 0.10 | 3.24 ± 0.27 | 0.433 ± 0.004 | 0.037 ± 0.002 | 5.27 ± 0.40 | |
CA11 | |||||||||
XR/XDHd | 48.3 ± 0.6 | 2.06 ± 1.51 | 7.38 ± 0.87 | 20.4 ± 1.3 | 1.11 ± 0.41 | 0.160 ± 0.022 | 0.442 ± 0.037 | 10.35 ± 3.55 | |
XR/XDH + XIe | 48.3 ± 0.6 | 0.574 ± 0.249 | 16.8 ± 0.5 | 5.67 ± 2.71 | 0.845 ± 0.265 | 0.352 ± 0.017 | 0.120 ± 0.059 | 10.05 ± 2.45 | |
XIf | 48.3 ± 0.6 | 0.135 ± 0.005 | 20.7 ± 0.46 | 2.26 ± 0.14 | 3.50 ± 0.05 | 0.430 ± 0.015 | 0.0469 ± 0.0036 | 6.55 ± 0.15 | |
PE-2∆GRE3 | 40 | ||||||||
XR/XDHa | 52.0 ± 0.8 | 17.8 ± 0.6 | 7.28 ± 0.43 | 13.1 ± 0.9 | 1.17 ± 0.01 | 0.213 ± 0.004 | 0.385 ± 0.042 | 1.60 ± 0.10 | |
XR/XDH + XIb | 52.0 ± 0.8 | 27.0 ± 1.5 | 4.67 ± 0.04 | 9.61 ± 0.94 | 1.15 ± 0.06 | 0.187 ± 0.007 | 0.386 ± 0.048 | 1.10 ± 0.10 | |
XIc | 52.0 ± 0.8 | 11.0 ± 0.7 | 16.4 ± 0.3 | 2.67 ± 0.04 | 3.51 ± 0.01 | 0.401 ± 0.006 | 0.0651 ± 0.0012 | 1.40 ± 0.10 | |
CA11 | |||||||||
XR/XDHd | 48.3 ± 0.6 | 19.9 ± 0.5 | 4.98 ± 0.35 | 12.3 ± 0.2 | 1.93 ± 0.03 | 0.175 ± 0.013 | 0.433 ± 0.005 | 3.65 ± 0.15 | |
XR/XDH + XIe | 48.3 ± 0.6 | 7.63 ± 0.14 | 14.1 ± 0.1 | 8.79 ± 0.34 | 2.07 ± 0.01 | 0.345 ± 0.006 | 0.216 ± 0.011 | 4.00 ± 0.80 | |
XIf | 48.3 ± 0.6 | 19.6 ± 0.7 | 10.6 ± 0.3 | 3.21 ± 0.17 | 4.42 ± 0.16 | 0.368 ± 0.008 | 0.112 ± 0.001 | 3.45 ± 0.05 | |