Skip to main content
Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: An efficient method for markerless mutant generation by allelic exchange in Clostridium acetobutylicum and Clostridium saccharobutylicum using suicide vectors

Fig. 1

Effect of lysozyme treatment on the transformation efficiency the C. saccharobutylicum Ch2 (∆hsdR1∆ hsdR2 ∆hsdR3) strain. Lysozyme (at a concentration of 150 μg/ml) was added in the culture medium when the A600 reached a value of 0.6 (see “Methods”). Incubation time varies between 5 and 50 min. The time point at t = 0 min correspond to an experiment without lysozyme added

Back to article page

Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

Contact us