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Table 1 Transformation efficiencies of C. acetobutylicum ∆cac1502 ∆cac3535 ∆upp ∆pSOL with unmethylated pCons2.1 plasmid

From: An efficient method for markerless mutant generation by allelic exchange in Clostridium acetobutylicum and Clostridium saccharobutylicum using suicide vectors

Lysozyme concentration (μg/ml)

Electroporation efficiencies

0

142 ± 47

15

648 ± 154

150

6.5 × 103 ± 2.2 × 103

1500

2.1 ± 0.2

  1. Values are expressed in number of transformants per µg of unmethylated pCons2.1
  2. Mean values and standard deviations from two independent experiments are given
  3. 5 µg pCons2.1 was used in each experiment
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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