An efficient method for markerless mutant generation by allelic exchange in Clostridium acetobutylicum and Clostridium saccharobutylicum using suicide vectors

Table 3 Solvent and acid formation by C. saccharobutylicum wild-type and mutant strains in batch culture without pH regulation

	C. saccharobutylicum wild type	C. saccharobutylicum ∆hsdR1∆hsdR2∆hsdR3∆upp	C. saccharobutylicum ∆hsdR1∆hsdR2∆hsdR3∆upp, spo0A*
[Acetone]_final (mM)	33	30	0
[Butanol]_final (mM)	83	76	0
[Ethanol]_final (mM)	11	9	6
[Acetate]_produced (mM)	11	15	28
[Butyrate]_final (mM)	12	16	47
Butanol yield (g g⁻¹)	0.17	0.16	0

Cultures were done at 37 °C in MES-MM medium supplemented with 0.001% yeast extract for 96 h

ISSN: 2731-3654