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Table 4 Heat resistance of spores from different C. acetobutylicum and C. saccharobutylicum strains

From: An efficient method for markerless mutant generation by allelic exchange in Clostridium acetobutylicum and Clostridium saccharobutylicum using suicide vectors

C. acetobutylicum ∆cac1502 ∆cac3535 ∆upp

C. saccharobutylicum ∆hsdR1∆hsdR2 ∆hsdR3 ∆upp

Control strain

Spo0A G179S

Control strain

Spo0A G172S

4 × 105

0

5 × 107

0

  1. 96 h cultures (in MES-MM medium supplemented with 0.001% yeast extract for C. saccharobutylicum and SM medium for C. acetobutylicum) were heat treated at 70 °C for 10 min. Values are expressed in number of CFU per ml of culture
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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