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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Enhancing co-translational folding of heterologous protein by deleting non-essential ribosomal proteins in Pichia pastoris

Fig. 3

Ribosome assembly in RP deletion strains and 60S processing factor deletion strains, analyzed by polysome profiles. a, b Polysome profiles of the “enhancing” a and “non-enhancing” b strains in relation to wild-type strain showed that the former had the lower 60S level. c Polysome profiles of RP deletion paralogs. The “enhancing” strain rps27a∆ showed a reduced level of 60S ribosomal subunits relative to its paralog deletion strain (“non-enhancing”). d, e Phy expressional profiles of non-essential 60S processing factor deletion strains relative to wild type. d Activity per OD600; e Growth curve. f Relative to wild type, polysome profiles for rei1Δ and nop12Δ strains showed reduction of the 60S subunit level

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