Fig. 2

Growth phenotypes in the presence of pretreatment-generated inhibitors and fermentation stresses. a The cells were grown in SD medium containing 2.0% glucose with varying concentration of pretreatment-generated inhibitors such as furfural (0.5 g/l, 1.0 g/l and 1.5 g/l), 5-HMF (1.0 g/l, 2.0 g/l and 3.0 g/l), acetic acid (0.2%, 0.3% and 0.4% v/v) and ethanol (6.0%, 8.0% and 10% v/v) at 40 °C. Relative growth in the presence of inhibitors was calculated by considering 100% growth in the absence of inhibitors. b Chemogenetic network profile: In silico Chemogenetic network profile was generated using Cytoscape 3.6.0 software using 1.5 g/l of furfural, 3.0 g/l of 5-HMF, 0.3% v/v of acetic acid and 10.0% v/v ethanol individually as well as in combinations. c Isolate NGY10 growth phenotypes in the presence of inhibitor: % growth reduction in presence of 1.0 g/l furfural, 3.0 g/l 5-HMF, 0.3% v/v acetic acid, 10% v/v ethanol, cocktail A (1.0 g/l furfural, 3.0 g/l 5-HMF, 0.3% v/v acetic acid and 10% v/v ethanol) and cocktail B (furfural: 0.618 g/l, 5-HMF: 0.748 g/l, acetic acid: 0.18% v/v and ethanol 5.0% v/v) as compared to without inhibitors in SD medium containing 2.0% glucose at 40 °C