Evaluation of divergent yeast genera for fermentation-associated stresses and identification of a robust sugarcane distillery waste isolate Saccharomyces cerevisiae NGY10 for lignocellulosic ethanol production in SHF and SSF

Pandey, Ajay Kumar; Kumar, Mohit; Kumari, Sonam; Kumari, Priya; Yusuf, Farnaz; Jakeer, Shaik; Naz, Sumera; Chandna, Piyush; Bhatnagar, Ishita; Gaur, Naseem A.

doi:10.1186/s13068-019-1379-x

Biotechnology for Biofuels and Bioproducts

Table 4 Fermentation performance of selected yeast isolates at 30 °C and 40 °C in SD media containing glucose/xylose

From: Evaluation of divergent yeast genera for fermentation-associated stresses and identification of a robust sugarcane distillery waste isolate Saccharomyces cerevisiae NGY10 for lignocellulosic ethanol production in SHF and SSF

S. no.	Yeast strains	30 °C						40 °C
S. no.	Yeast strains	Ethanol concentration (g/l)	Ethanol yield (g/g)	Ethanol productivity (g/l//h)	Ethanol efficiency (%)	Glycerol concentration (g/l)	Acetic acid concentration (g/l)	Ethanol concentration (g/l)	Ethanol yield (g/g)	Ethanol productivity (g/l/h)	Ethanol efficiency (%)	Glycerol concentration (g/l)	Acetic acid concentration (g/l)
Initial sugar = glucose (100.0 g/l)
1.	Angel yeast	48.94 ± 1.38^a	0.489	2.04	95.69	1.72	0.314	45.91 ± 1.45	0.471	1.91.2	92.17	4.11	0.361
2.	S. cerevisiae CEN-PK-122	48.79 ± 0.61	0.488	2.032	95.5	2.414	0.624	41.58 ± 1.08	0.43	1.732	84.148	2.722	0.936
3.	S. cerevisiae NGY1	47.59 ± 2.34	0.485	1.983	94.91	3.744	0.37	45.65 ± 2.44	0.47	1.902	91.976	4.374	0.464
4.	S. cerevisiae NGY10	49.77 ± 0.34*	0.497	2.073	97.397	4.733	0.377	46.81 ± 3.11*	0.478	1.950	93.542	5.206	0.421
5.	K. marxianus NCIM 3465	42.19 ± 3.08	0.48	1.757	93.933	3.945	0.476	41.22 ± 2.87	0.45	1.7175	88.062	4.016	0.465
6.	K. marxianus NGY8	46.50 ± 0.99	0.473	1.937	92.563	2.379	0.395	42.46 ± 2.00	0.461	1.769	90.215	3.025	0.395
7.	C. glabrata CBS138	43.63 ± 2.23	0.472	1.817	92.367	3.647	0.323	42.72 ± 2.55	0.459	1.78	89.823	4.276	0.316
8.	C. glabrata NGY7	47.81 ± 2.11	0.48	1.992	93.933	4.705	0.426	46.09 ± 1.44	0.46	1.92	90.02	3.406	0.447
9.	P. kudriavzevii NGY12	45.63 ± 2.71	0.469	1.901	91.78	2.364	0.339	43.60 ± 1.88	0.455	1.816	89.04	2.064	0.149
10.	P. kudriavzevii NGY20	43.44 ± 1.38	0.47	1.81	91.976	2.161	0.317	42.12 ± 1.45	0.462	1.755	90.41	2.173	0.344
11.	C. tropicalis NGY19	41.49 ± 1.96	0.43	1.81	84.15	2.161	0.317	41.77 ± 1.98	0.429	1.740	84.0	0.918	0.447
Initial sugar = xylose (50.0 g/l)
12.	S. stipitis NCIM3507	6.038 ± 0.31	0.416	0.251	81.321	0.037	0.045	3.37 ± 0.128	0.32	0.14	62.63	0.037	0.045
13.	S. stipitis NCIM3498	6.393 ± 0.27	0.439	0.266	86.1	0.021	0.05	3.75 ± 0.133	0.325	0.156	63.625	0.021	0.05
14.	K. marxianus NGY8	1.525 ± 0.013	0.359	0.063	70.385	0.048	0.033	1.48 ± 0.025	0.349	0.061	68.31	0.048	0.033
15.	C. shehatae NCIM 3500	5.821 ± 0.02	0.320	0.242	62.765	1.11	0.311	3.74 ± 0.016	0.284	0.155	55.58	1.11	0.311
16.	C. lusitaniae NCIM 3484	1.509 ± 0.01	0.444	0.062	86.930	0.005	0.082	0.601 ± 0.023	0.430	0.025	84.189	0.005	0.082
17.	W. anomalus NGY2	0.704 ± 0.03	0.159	0.029	31.155	0.028	0.046	0.614 ± 0.019	0.138	0.025	27.172	0.028	0.046
18.	O. thermophila NGY11	0.445 ± 0.03	0.089	0.018	17.564	0.002	0.17	0.312 ± 0.013	0.021	0.013	4.11	0.002	0.17
19.	C. glabrata NGY14	0.11 ± 0.006	0.141	0.005	27.597	0.002	0.03	0.06 ± 0.005	0.005	0.0025	0.98	0.003	0.01
20.	P. kudriavzevii NGY12	0.544 ± 0.025	0.405	0.023	79.446	0.001	0.01	0.444 ± 0.025	0.189	0.018	37.131	0.001	0.01
21.	C. dubliniensis NGY5	0.535 ± 0.03	0.137	0.022	26.914	0.093	0.09	0.211 ± 0.004	0.003	0.008	0.0	0.093	0.09
22.	C. tropicalis NGY22	0.794 ± 0.03	0.235	0.033	46.120	0.12	0.071	0.541 ± 0.028	0.228	0.022	44.690	0.12	0.071

^aMean ± standard deviation, n = 3; Fermentation volume: 50 ml; pH-5.4; inoculums 5.0% v/v ≈ 1.0 × 10⁷ cells/ml, fermentation time 24 h
* Statistical student t-test of isolate NGY10 with reference strains CEN-PK-122 and Angel yeast for glucose fermentation at 30 °C and 40 °C was performed, and show significance, i.e. p < 0.05

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