Fig. 2

Assessment of C. glutamicum Resistance to Three Classes of ILs and Exogenous Isopentenol. a–c Growth assay for assessment of C. glutamicum resistance to ILs: C. glutamicum was cultured in CGXII minimal medium containing 4% d-glucose and the type and concentration of IL as indicated. Strains were cultivated in a 96 well microtiter plate. The growth rate in either control media or supplemented with exogenous IL as indicated (1/h) was calculated and plotted as a function of IL concentration. Data is the average of three independent biological replicates, and error bars indicate standard error. d Spot assay for assessment of C. glutamicum and E. coli resistance to isopentenol. Cells were serially diluted tenfold onto solid LB agar media with or without 0.2% (w/v) isopentenol as indicated and incubated at 30 °C. Error bars are not plotted when they are shorter than the symbol used on the graph. Photomicrographs were taken after plates were incubated for 2 days