Fig. 8

Analysis of HiPMO1 reaction products hydrolyzed by beta-glucuronidase and beta-glucosidase using HPAEC–PAD analysis. a Qualitative analysis: HiPMO1–HiPMO1 reaction products hydrolyzed by beta-glucuronidase and beta-glucosidase; CK-the control sample analyzed as above except without HiPMO1; standard glucose, gluconic acid, glucuronic acid, and saccharic acid. HPAEC–PAD analysis of a mixture of equal amounts of standard glucose, gluconic acid, glucuronic acid, and saccharic acid shows that saccharic acid has the lowest sensitivity, resulting in the minor peak corresponding to saccharic acid in HiPMO1 reaction products hydrolyzed by beta-glucuronidase and beta-glucosidase. b Quantitative analysis: the rate of formation of gluconic acid, glucuronic acid, and saccharic acid in HiPMO1 reaction products hydrolyzed with beta-glucuronidase and beta-glucosidase were quantified from standard curves of gluconic acid, glucuronic acid, and saccharic acid using HPAEC–PAD analysis. The unit of the rate was expressed in µg/µM h, the amount (µg) of product formed per enzyme molecule (µM) per unit time (h). The control sample analyzed as above except without HiPMO1 (CK)