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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: AA16, a new lytic polysaccharide monooxygenase family identified in fungal secretomes

Fig. 4

Oxidative cleavage of cellulose by AaAA16. a HPAEC–PAD chromatograms showing soluble products generated from 0.1% PASC cellulose using 4.4 µM AaAA16, with or without l-cysteine (1 mM). The peak annotations are based on comparison with oligosaccharides standards, native (DP1–DP5) or oxidized at the C1 position (DP2ox-DP4ox). The box at the right top shows an enlargement of the C1-oxidized product region. b MS spectrum of soluble degradation products generated by AaAA16 from PASC. Mass spectrometry analysis of soluble degradation products generated by AaAA16 from PASC. The main panel shows the full-scale spectrum of the sample containing native and oxidized cello-oligosaccharides, and the boxed regions are magnified to show the peaks corresponding to oxidized cello-oligosaccharides. c MS/MS spectrum of the DP3 oxidized product peak (m/z 519.16) observed on (b). The fragmentation pattern corresponds to a C1 oxidized species with an aldonic acid at the reducing end. Observed fragments are depicted on the structure. Blue triangles: Water losses. Black stars: contaminant peak from an ion co-isolated during the MS/MS precursor selection

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