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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: CRISPR–Cas ribonucleoprotein mediated homology-directed repair for efficient targeted genome editing in microalgae Nannochloropsis oceanica IMET1

Fig. 2

a Percent of mutants obtained with various RNP-based HDR. b Gel electrophoresis image of the WT, NR-KO and mixed colonies upon colony PCR. c Phenotypic characterization of NR mutants, the NR mutants were observed to bleach in media with nitrate as the sole nitrogen source while they grew similar to WT in media with ammonia as the nitrogen source (The re-streak plates for characterization included in Additional file 1: Data S13). d Sequencing result of a NR-KO mutant showing precise integration of the Zeocin resistance cassette into the host genome knocking out the NR gene

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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