Fig. 6

Co-overexpression of HRK1/ScACS1*, HRK1/PpACS1 for 6-MSA biosynthesis. Cell growth (a), 6-MSA production (b) and 6-MSA productivity (c) of recombinant strains XN, XN-HRK1-PpACS1 and XN-HRK1-ScACS1* in medium with acetate of 0, 20 and 30 mM, respectively. Culture conditions were as same as that described in Fig. 3. Acetate concentrations were marked on top of the figure. Acetate concentrations were marked the same as those in Fig. 3. Statistical significance of 6-MSA production and productivity by overexpression of PpACS1, ScACS1* and pta/ackA relative to the parent strain of XN at each time point is also shown. **P < 0.01, *P < 0.05 at 24 h; ^##P < 0.01, ^#P < 0.05 at 48 h; ^⁜⁜P < 0.01, ^⁜P < 0.05 at 72 h; N.S., not significance. n = 6 or 9 for each strain at specific time points. The error bars represent the standard deviation of three biological replicates assayed in duplicate or triplicate