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Table 1 Comparison of fermentation characteristics of butyrate-producing E. coli

From: Metabolic engineering of Escherichia coli for the production of butyric acid at high titer and productivity

Pathwaya Mediumb Batch/fed-batch Titer (g l−1) Yield (g g−1)c Productivityd Comment References
Thioesterase TB Batch 9.7   [25]
Thioesterase Modified TB Batch 4.4 0.49 0.18   [26]
Thioesterase Minimal + YE Batch
Fed-batch
7.6
12.3

0.31

0.23
Aerated
Aerated
[22]
Thioesterase TB Batch
Fed-batch
4.3
7.20
0.43

  [20]
PTB/BUK; BCD Minimal Batch 0.3   [19]
CoA transferase Modified TB Batch
Fed-batch
6.8
10.0


+ acetate [23]
FA pathway + CoA transferase LB Batch 1.0   [24]
FA pathway + thioesterase Minimal
Minimal
Batch
Fed-Batch
0.8
4.6
0.11

Aerated
Aerated
[21]
PTB/BUKe Minimal + YE Batch 33.1 ± 0.3 0.37 ± 0.01 0.89 ± 0.07   This study
  1. –, Data not available
  2. aExcept for the FA (fatty acid) pathway, all other butyrate pathways utilized the basic Clostridium pathway with Ter substituting for BCD complex, unless stated otherwise, to butyryl-CoA followed by the indicated enzyme(s) for the conversion of butyryl-CoA to butyrate (Fig. 1). PTB, phosphotransbutyrylase; BUK, butyrate kinase; BCD, butyryl-CoA dehydrogenase complex
  3. bTB, terrific broth; Minimal, mineral salt medium; YE, yeast extract; LB, Luria broth
  4. cYield—g butyrate.(g sugar consumed)−1
  5. dProductivity—volumetric productivity is expressed as g l−1 h−1 and the values reported from this study are average with standard deviation over 24 h from three independent experiments
  6. eValues from this study are from glucose- or xylose-mineral salt medium with yeast extract (5 g l−1) and are the average and standard deviation from three independent experiments. See text for details