Fig. 5

Correlation analysis of the mutants of 7421ADO. a, b The relative amount of total hydrocarbon produced in E. coli coexpressing 7942AAR and a mutant of 7421ADO plotted against the relative amount of soluble ADO (a) and relative activity for total hydrocarbons (b). c, d The relative amount of soluble 7421ADO plotted against the solubility (c) and relative protein expression level of 7421ADO (d). e, f The relative activity for total hydrocarbons of 7421ADO plotted against the relative amount of soluble 7421ADO (e) and solubility (f). In each panel, the red continuous line indicates a linear regression, and the corresponding correlation coefficient, r, and p value are shown. In c, f, the data point for R181E is highlighted. The blue dotted line indicates a linear regression obtained without using the data for R181E