Fig. 1

Overview of TfAA10A expression in Synechococcus elongatus UTEX 2973. a Overview of TfAA10A expression cassettes inserted into the pDF-trc self-replicating plasmid used for S. elongatus UTEX 2973 transformation. TfAA10A encodes the native protein sequence. For TorA-TfAA10A the N-terminal native signal peptide was removed and replaced by the E. coli TorA signal peptide. In both constructs, a C-terminal human influenza hemagglutinin (HA) epitope tag was added to the protein for detection purposes. b Cellular lysates of two independent transformants expressing TfAA10A and TorA-TfAA10A detected by immunoblot analysis using an HA antibody (αHA). Pre- and mature (processed) protein bands are indicated with arrows. Ctrl indicates an empty pDF-trc vector control strain. Lanes are normalized to 10 µg of total protein. A Coomassie-stained polyacrylamide gel is shown as a loading control. An αRbcL blot is shown as a cell lysis control. c Cell-free culture medium of two independent transformants expressing TfAA10A and TorA-TfAA10A detected by immunoblot analysis using αHA. The mature (processed) protein band is indicated with an arrow. Ctrl indicates an empty vector control strain. Lanes are normalized to 2 mL of cell-free culture medium. A Coomassie-stained polyacrylamide gel is shown as a loading control. An αRbcL blot is shown as a cell lysis control