Fig. 3

Detail of the glycome profile of leaf and stem cell wall biomass from 4 miscanthus genotypes, using 21 of the 155 glycan-directed mAbs. Cell wall fractionation was achieved by sequentially employing ammonium oxalate, AO; sodium carbonate, CA; 1 M KOH, 1 M; 4 M KOH, 4 M; sodium chlorite, CH and 4 M KOH post-chlorite treatment, PC. Clear differences are visible in cell wall glycans between the various genotypes, at identical developmental stages and organs. The different colours of the mAb names to the right refer to their glycan-binding subclasses, which are further elucidated elsewhere [28, 30,31,32] and in Additional file 1. Antibody binding strength based on optical density (OD) is presented as a colour gradient ranging from dark blue (no binding: OD = 0.0) and red (intermediate binding) to yellow (strongest binding: OD = 1.3). For complete glycome profiles for all tested genotypes and 155 mAbs, including the amounts of extracted carbohydrate in each sequential extract, refer to Additional file 5. PB, peak biomass; and SS, senesced stages; XG, xyloglucan; MLG, mixed-linkage glucan; HG, homogalacturonan; RG-I, rhamnogalacturonan-I; AG, arabinogalactan; RG-I/AG, AG side chains of RG-I