Fig. 3

Fitness test of TRK1 mutants treated with 35 mM PA. Three different TRK1 point mutations were reverse engineered into the parental strain CEN.PK 113-7D to generate the TRK1^H1074N, TRK1^R993P, and TRK1^A1169E strains. Growth rates of these three strains carrying TRK1 point mutations, the end-point PA-evolved isolate PA-1 (evolved), and the parental strain were determined in buffered minimal medium containing 35 mM PA (a). Growth rates of a TRK1 deletion strain (trk1Δ), a TRK1 overexpression strain (TRK1-OVER) and the parental strain were measured in buffered minimal medium containing 35 mM PA (b). Bars and error bars represent the mean and SD of triplicate cultures