Fig. 1

Triggering glutamic acid accumulation by C-terminal truncation of MscCG and bioY knockout of C. glutamicum S9114. a Cell growth; b glucose consumption; c glutamic acid accumulation. Parental indicates the original C. glutamicum S9114 strain; ΔC110 indicates the strain with the MscCG C-terminal truncated; ΔbioY indicates the strain with bioY gene knocked out; ΔC110ΔbioY indicates the strain with the MscCG C-terminal truncated and bioY gene knocked out. Glutamic acid fermentations were carried out in 3-L fermentor (3BG-4, Baoxing Biotech Co., Shanghai, China) at 32 °C, 1.4 vvm of aeration and 600 rpm. No penicillin was added for induction. Mean values are presented with error bars representing the minimum and maximum values