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Table 1 RBS sequence and predicted translation initiation rate of odhA

From: Engineering Corynebacterium glutamicum triggers glutamic acid accumulation in biotin-rich corn stover hydrolysate

Name

Sequence (5′–3′)a

Predicted translation initiation rate (a.u.)

Original

CAAGGAAAAGAGGCGAGTACCTGCC

50.38

RBS20

GCTAAATATCATACCGATAAAGTCATA

20.72

RBS10

GGTCCCTCAAGTTAACCACGCGGC

10.33

RBS0.1

CTCACCCACGAGTTCAATAACTAGG

0.11

  1. aThe strength prediction of original RBS sequence of odhA and the design of RBS sequence with different strength are carried out by RBS Calculator (https://www.denovodna.com/software/doLogin)
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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