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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Enhancement of lignocellulosic degradation in high-moisture alfalfa via anaerobic bioprocess of engineered Lactococcus lactis with the function of secreting cellulase

Fig. 3

SDS-PAGE of the recombinant strains. a M, protein molecular weight marker; lane a, intracellular protein of L. lactis subsp. lactis MG1363; lanes b and c, intracellular protein of L. lactis subsp. lactis MG1363/pMG36e-usp45-egl3; lane d, intracellular protein of L. lactis subsp. lactis MG1363/pMG36e; lane e, extracellular protein of L. lactis subsp. lactis MG1363/pMG36e; lanes f and g, extracellular protein of L. lactis subsp. lactis MG1363/pMG36e-usp45-egl3; b lanes 1 and 2, extracellular protein of L. lactis subsp. lactis MG1363/pMG36e-usp45-cbh2; lanes 3 and 4, intracellular protein of L. lactis subsp. lactis MG1363/pMG36e-usp45-cbh2; lanes 5 and 6, intracellular protein of L. lactis subsp. lactis MG1363/pMG36e; lanes 7 and 8, extracellular protein of L. lactis subsp. lactis MG1363/pMG36e; lanes 9 and 10, extracellular protein of L. lactis subsp. lactis MG1363/pMG36e-usp45-bgl1; lanes 11 and 12, intracellular protein of L. lactis subsp. lactis MG1363/pMG36e-usp45-bgl1; M, protein molecular weight marker

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