Fig. 3

Inverted phylogenetic tree based on traditional multiple alignment of all GH30 protein domains. The numbering of the CUPP groups (numbered 1 to 33) is indicated directly on the tree. Each entry has a colored square indicating the CUPP group to which it belongs (black entries were ignored during CUPP clustering). Inside the tree are six numbered rings: the outermost ring, ring 1, indicates subfamily delineation according to CAZy and adjacent label of subfamilies; ring 2 is the subfamilies predicted by the dbCAN tool; and ring 3 and ring 4 show, respectively, the subfamily assigned by CUPP clustering and by CUPP prediction. Ring 5 and 6 represent the EC functions annotated by CUPP prediction for each entry as a result of CUPP clustering (ring 5) and of CUPP prediction (ring 6). White color (= empty spaces) in a ring indicates that no specific relation to subfamily or function could be assigned. The entries with functional annotation by CAZy are indicated by their respective EC numbers. The circles in the center indicates taxonomic groups where blue refers to bacterial, olive green refers to fungal, and lime refers to nonfungal eukaryote, while white refers to unknown taxonomy. An interactive version of the tree is available online: https://itol.embl.de/tree/1302256425066571528733948