Fig. 8
The PoxCxrA17–150-binding sequence in the promoter region of transcription factor gene PoxClrB. a Schematic diagram of putative PBM_PoxClrB truncation. b The PBM_PoxClrB sequence determined by in vitro EMSA. c EMSA analysis of key nucleotides in PBM_PoxClrB. Each EMSA experiments comprised 0–2 µg of PoxCxrA17–150 and 50 ng of truncated PBM_PoxClrB as probe labelled with 6-carboxyfluorescein. The promoter of β-tubulin gene POX05989 was used as a probe control. The fusion protein Trx–His–S purified from E. coli cells harbouring the empty plasmid pET32a (+), and BSA alone, were used as protein controls