Fig. 1

SDS-PAGE analysis of the high-molecular-weight cellulosomal fractions. B. cellulosolvens cellulosomal fractions, derived from cellobiose- and microcrystalline cellulose-grown cells, were separated by gel filtration (see Additional file 2: Figure S1). Each spent-cell medium (concentrated supernatant fluids) is represented by two peaks—I and II. The cellulosomes (20 µg) were subjected to 4-to-15% gradient SDS-PAGE. CB: cellobiose; MCC: microcrystalline cellulose; Ct: purified C. thermocellum cellulosome