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Fig. 5 | Biotechnology for Biofuels

Fig. 5

From: N-terminal domain replacement changes an archaeal monoacylglycerol lipase into a triacylglycerol lipase

Fig. 5

Biochemical characterization of immobilized enzymes. a Relative activity of immobilized TON-LPL (black) and rc-TGL (gray) with different substrates pNP C-2 (acetate); pNP C-4 (butyrate); pNP C-8 (octanoate), pNP C-12 (dodecanoate), and pNP-C-16 (palmitate). Here the enzyme units are expressed in per gram of resin having bound enzymes. b HPLC-based quantification of ricinoleic acid (mg/ml) produced by hydrolysis of castor oil. The standard curve or ricinoleic acid and HPLC profiles of castor oil hydrolysis are shown in Additional file 6

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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