Table 3 Experimental conditions and results of central composite design (2 3-level factors, 3 blocks, 9 run) with n = 3 for each assay
From: Species disparity response to mutagenesis of marine yeasts for the potential production of biodiesel
Experiment | Variables | Response | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
YlTun15 | TaTun15 | RmTun15 | DhTun2015 | CtTun15 | ||||||||
EMS [mM] | Volume EMS (ml) | Colony (CFU) | OD (600Â nm) | Colony (CFU) | OD (600Â nm) | Colony (CFU) | OD (600Â nm) | Colony (CFU) | OD (600Â nm) | Colony (CFU) | OD (600Â nm) | |
1 | 75 | 100 | 23 | 19.41 | 19 | 21.9 | 33 | 19.44 | 0 | 0 | 0 | 0 |
2 | 100 | 50 | 21 | 20.16 | 29 | 21.26 | 31 | 19.57 | 5 | 15.68 | 0 | 0 |
3 | 25 | 15 | 182 | 20.12 | 222 | 21 | 287 | 15.38 | 200 | 13.95 | 191 | 16.36 |
4 | 25 | 50 | 163 | 20.47 | 187 | 21.9 | 217 | 16.12 | 171 | 18.12 | 163 | 17.68 |
5 | 100 | 100 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
6 | 75 | 15 | 175 | 20.14 | 193 | 21.18 | 272 | 20.94 | 173 | 19.63 | 97 | 24.68 |
7 | 100 | 15 | 81 | 18.95 | 161 | 19.38 | 117 | 16.5 | 63 | 14.21 | 48 | 23.15 |
8 | 25 | 100 | 79 | 19.48 | 130 | 20.38 | 121 | 18.44 | 94 | 20.31 | 87 | 20.65 |
9 | 75 | 50 | 89 | 21.8 | 95 | 22.41 | 132 | 22.82 | 82 | 21.31 | 13 | 25.53 |