Fig. 4From: Synthetic biology for evolutionary engineering: from perturbation of genotype to acquisition of desired phenotypeGenetic cassette designs being applied for evolution of protein functions with PACE. a Bt toxin evolution to targeting new receptor. b TEV protease evolution to target new cleavage site. c aaRS evolution to get specificity with ncAA and suppressor tRNA. d Eukaryotic protein evolution to improve solubility in E. coli. e Cas9 evolution to recognize new PAMBack to article page