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Table 1 List of helper enzymes

From: Synthetic biology for evolutionary engineering: from perturbation of genotype to acquisition of desired phenotype

Target product Regulatory protein/detection method Helper enzyme Detectable chemical References
3-HP PrpR (Escherichia coli) Pcs, PrpC 2-Methylcitrate [71]
3-HP AcuR (Rhodobacter sphaeroides) pcs mutant, ach Acrylate [71]
1,3-PDO MmsR (Pseudomonas denitrificans) KgsA mutant 3-HP [72]
ω-Amino fatty acids NitR (Alcaligenes faecalis) Cyclase Lactam [73]
Tyrosine Dark pigment MelA (Rhizobium etli) Melanin [74]
Malonyl-CoA Colorimetric indicator RppA (Streptomyces griseus) FLAVIOLIN [75]