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Table 1 List of helper enzymes

From: Synthetic biology for evolutionary engineering: from perturbation of genotype to acquisition of desired phenotype

Target product

Regulatory protein/detection method

Helper enzyme

Detectable chemical

References

3-HP

PrpR (Escherichia coli)

Pcs, PrpC

2-Methylcitrate

[71]

3-HP

AcuR (Rhodobacter sphaeroides)

pcs mutant, ach

Acrylate

[71]

1,3-PDO

MmsR (Pseudomonas denitrificans)

KgsA mutant

3-HP

[72]

ω-Amino fatty acids

NitR (Alcaligenes faecalis)

Cyclase

Lactam

[73]

Tyrosine

Dark pigment

MelA (Rhizobium etli)

Melanin

[74]

Malonyl-CoA

Colorimetric indicator

RppA (Streptomyces griseus)

FLAVIOLIN

[75]