Fig. 2

Characterization of transformants of CrCAO. a PCR detection of the inserted plasmid (pNsChlb) by amplifying the 375 bp Shble sequence only in transformants NsChlb7 and NsChlb19. The 380 bp product of 18S rDNA was used as a loading control and was detected in all samples. b Western blotting for expression of the FLAG-tagged CrCAO protein from transformants. The Flag tag was detected only in transformants, while the β subunit of ATP synthase (Atpβ) appeared as the F-type H-ATPase β subunit (72.6 kDa) and the CF1 β subunit (53.13 kDa) in WT and transformants. c Detection of chlorophylls and other photosynthetic pigments via HPLC at 460 nm absorbance. Peaks for chlorophyll a and a few carotenoids including β-carotene and violaxanthin were shown. Emergence of chlorophyll b was detected only in the transformants