Heterologous synthesis of chlorophyll b in Nannochloropsis salina enhances growth and lipid production by increasing photosynthetic efficiency

Table 2 Lipid contents of WT and NsChlb transformants grown under different light conditions measured by HPLC

	Strain	TAG	MAG	MGDG	DGDG	Total
ML (90 μE)
Day 8	WT	3.90 ± 0.14	n.d.	7.77 ± 0.13	2.33 ± 0.05	14.00 ± 0.13
	NsChlb 7	3.57 ± 0.27	n.d.	7.64 ± 0.55	2.56 ± 0.09 *	13.77 ± 0.54
	NsChlb 19	4.93 ± 0.52	n.d.	7.40 ± 0.21	2.79 ± 0.19 *	15.12 ± 0.80
Day 12	WT	3.03 ± 0.08	2.07 ± 0.02	5.05 ± 0.32	2.28 ± 0.02	14.21 ± 0.31
	NsChlb 7	2.76 ± 0.06*	2.01 ± 0.02	6.30 ± 0.29*	2.47 ± 0.03**	14.37 ± 0.95
	NsChlb 19	3.06 ± 0.27	2.01 ± 0.01	6.12 ± 0.19*	2.49 ± 0.05**	15.67 ± 0.69
HL (200 μE)
Day 8	WT	4.58 ± 0.62	2.89 ± 0.03	3.71 ± 0.06	2.55 ± 0.02	14.01 ± 0.81
	NsChlb 7	5.45 ± 0.56	3.17 ± 0.13	3.62 ± 0.02	2.59 ± 0.06	15.10 ± 0.70
	NsChlb 19	6.98 ± 0.81*	2.84 ± 0.02	3.44 ± 0.13	2.60 ± 0.10	15.94 ± 0.53
Day 12	WT	32.32 ± 1.41	2.51 ± 0.02	2.04 ± 0.05	1.80 ± 0.04	38.67 ± 1.33
	NsChlb 7	31.68 ± 2.14	2.52 ± 0.07	2.06 ± 0.10	1.82 ± 0.07	38.08 ± 1.92
	NsChlb 19	31.43 ± 1.77	2.43 ± 0.03	2.04 ± 0.04	1.78 ± 0.02	37.67 ± 1.71

The data represent the average value with ranges of standard error (n = 3). Asterisks indicates the significant differences between WT and each transformants (WT vs. NsChlb7, WT vs. NsChlb19) determined by Student’s t test (*P < 0.05, **P < 0.01, ***P < 0.001)