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Table 2 Lipid contents of WT and NsChlb transformants grown under different light conditions measured by HPLC

From: Heterologous synthesis of chlorophyll b in Nannochloropsis salina enhances growth and lipid production by increasing photosynthetic efficiency

  Strain TAG MAG MGDG DGDG Total
ML (90 μE)
 Day 8 WT 3.90 ± 0.14 n.d. 7.77 ± 0.13 2.33 ± 0.05 14.00 ± 0.13
NsChlb 7 3.57 ± 0.27 n.d. 7.64 ± 0.55 2.56 ± 0.09 * 13.77 ± 0.54
NsChlb 19 4.93 ± 0.52 n.d. 7.40 ± 0.21 2.79 ± 0.19 * 15.12 ± 0.80
 Day 12 WT 3.03 ± 0.08 2.07 ± 0.02 5.05 ± 0.32 2.28 ± 0.02 14.21 ± 0.31
NsChlb 7 2.76 ± 0.06* 2.01 ± 0.02 6.30 ± 0.29* 2.47 ± 0.03** 14.37 ± 0.95
NsChlb 19 3.06 ± 0.27 2.01 ± 0.01 6.12 ± 0.19* 2.49 ± 0.05** 15.67 ± 0.69
HL (200 μE)
 Day 8 WT 4.58 ± 0.62 2.89 ± 0.03 3.71 ± 0.06 2.55 ± 0.02 14.01 ± 0.81
NsChlb 7 5.45 ± 0.56 3.17 ± 0.13 3.62 ± 0.02 2.59 ± 0.06 15.10 ± 0.70
NsChlb 19 6.98 ± 0.81* 2.84 ± 0.02 3.44 ± 0.13 2.60 ± 0.10 15.94 ± 0.53
 Day 12 WT 32.32 ± 1.41 2.51 ± 0.02 2.04 ± 0.05 1.80 ± 0.04 38.67 ± 1.33
NsChlb 7 31.68 ± 2.14 2.52 ± 0.07 2.06 ± 0.10 1.82 ± 0.07 38.08 ± 1.92
NsChlb 19 31.43 ± 1.77 2.43 ± 0.03 2.04 ± 0.04 1.78 ± 0.02 37.67 ± 1.71
  1. The data represent the average value with ranges of standard error (n = 3). Asterisks indicates the significant differences between WT and each transformants (WT vs. NsChlb7, WT vs. NsChlb19) determined by Student’s t test (*P < 0.05, **P < 0.01, ***P < 0.001)