Molecular evolutionary engineering of xylose isomerase to improve its catalytic activity and performance of micro-aerobic glucose/xylose co-fermentation in Saccharomyces cerevisiae

Table 1 Comparison of specific aerobic growth rates in YPX₅₀ medium for strains harboring XIs

Strain	Name	Origin	Specific growth rates (µ) (h⁻¹)
SS36	^a	^a	NA
SS37	BcXI	Burkholderia cenocepacia J2315	NA
SS38	PrXI	Prevotella aff. ruminicola TC2-24	0.39 ± 0.05
SS39	RfXI	Ruminococcus flavefaciens 17	0.58 ± 0.03
SS40	OspXI	Orpinomyces sp. ukk1	0.71 ± 0.07
SS41	PspXI	Piromyces sp. E2	0.51 ± 0.10
SS42	LpXI	Lachnoclostridium phytofermentans ISDg	0.62 ± 0.09
SS43	RcXI	Ruminiclostridium cellulolyticum H10	NA
SS44	SrXI	Streptomyces rubiginosus	0.03 ± 0.01

The mean specific growth rate with standard deviations of biological triplicates are shown
NA: aerobic growth in YPX₅₀ medium was very poor or showed no growth
^aSS36 was a control strain carrying a plasmid vector lacking any XI genes

ISSN: 2731-3654