Molecular evolutionary engineering of xylose isomerase to improve its catalytic activity and performance of micro-aerobic glucose/xylose co-fermentation in Saccharomyces cerevisiae

Table 2 Mutations identified in LpXI-expressing plasmids and relative specific growth rate (µ/µ₀) of isolated clones in YPX₈₀ medium under aerobic cultivations

Clone	Substitutions in LpXI	Specific growth rate (µ) (h⁻¹)^a	Relative growth rate (µ/µ₀)
M6-13	N223I	1.07 ± 0.06	3.97
M6-11	D207G	0.98 ± 0.12	3.63
M6-21	R191K/E192K	0.95 ± 0.11	3.54
M6-22	L304S	0.93 ± 0.09	3.44
M6-15	L78S	0.92 ± 0.18	3.43
M6-7	Y13H/D228 V/P233P	0.86 ± 0.16	3.20
M6-10	T273A	0.82 ± 0.07	3.06
M6-6	T133T/K136T/A176T	0.82 ± 0.31	3.04
M6-19	E114G	0.75 ± 0.08	2.80
M6-20	V162A/T273T/N303T	0.49 ± 0.03	1.81
M6-2	T63I/A121A	0.30 ± 0.03	1.13
SS42	Wild-type	0.27 ± 0.00	1.00

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