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Table 2 Mutations identified in LpXI-expressing plasmids and relative specific growth rate (µ/µ0) of isolated clones in YPX80 medium under aerobic cultivations

From: Molecular evolutionary engineering of xylose isomerase to improve its catalytic activity and performance of micro-aerobic glucose/xylose co-fermentation in Saccharomyces cerevisiae

Clone Substitutions in LpXI Specific growth rate (µ) (h−1)a Relative growth rate (µ/µ0)
M6-13 N223I 1.07 ± 0.06 3.97
M6-11 D207G 0.98 ± 0.12 3.63
M6-21 R191K/E192K 0.95 ± 0.11 3.54
M6-22 L304S 0.93 ± 0.09 3.44
M6-15 L78S 0.92 ± 0.18 3.43
M6-7 Y13H/D228 V/P233P 0.86 ± 0.16 3.20
M6-10 T273A 0.82 ± 0.07 3.06
M6-6 T133T/K136T/A176T 0.82 ± 0.31 3.04
M6-19 E114G 0.75 ± 0.08 2.80
M6-20 V162A/T273T/N303T 0.49 ± 0.03 1.81
M6-2 T63I/A121A 0.30 ± 0.03 1.13
SS42 Wild-type 0.27 ± 0.00 1.00
  1. aMean ± standard deviations