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Table 2 Lipid class composition of R. toruloides presented as percentage of total identified lipids

From: Proteome analysis of xylose metabolism in Rhodotorula toruloides during lipid production

 

t1

t3

Glucose

Xylose

Glucose

Xylose

% of lipid classes

mg/gDCW

% of lipid classes

mg/gDCW

% of lipid classes

mg/gDCW

% of lipid classes

mg/gDCW

Phospholipids

15.09 ± 0.34

13.71

16.27 ± 1.5

17.56

4.11 ± 0.34

19.53

3.9 ± 0.21

18.37

Monoacylglycerols

1.02 ± 0.33

0.93

1.35 ± 0.5

1.46

nd

nd

nd

nd

Diglycerols

8.35 ± 0.7

7.59

7.88 ± 0.26

8.50

4.9 ± 0.3

23.28

4.02 ± 0.36

18.93

Sterols

14.11 ± 0.34

12.82

14.61 ± 0.79

15.76

6.49 ± 0.3

30.83

5.67 ± 0.15

26.71

Free fatty acids

38.15 ± 0.9

34.66

40.77 ± 3.1

43.99

11.9 ± 0.3

56.54

10.80 ± 0.25

50.87

Triacylglycerols

23.82 ± 1.7

21.64

19.39 ± 4.5

20.92

72.64 ± 0.76

345.11

75.61 ± 0.63

356.12

TAG/PL

1.58

 

1.19

 

17.66

 

19.37

 

Total lipid content

9.085 ± 1

 

10.79 ± 1

 

47.51 ± 1.8

 

47.1 ± 1.4

  1. Samples were collected during early exponential phase (t1) and lipid production phase (t3). Total lipids were not determined at t1, but instead the values reported here are the mean total lipid contents between the first two total lipid measurements (Fig. 1), assuming a linear increase over time. Absolute levels of lipid classes were determined from the mean percentages of lipid classes and mean value of total lipid content
  2. DCW dry cell weight, TAG triacylglycerols, PL phospholipids
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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