Biochemical profiling, prediction of total lipid content and fatty acid profile in oleaginous yeasts by FTIR spectroscopy

Table 2 Tentative peak assignment of spectral bands in FTIR spectra of fungi [20]

Peak nr.	Frequency (cm⁻¹)	Peak assignment	Main biomolecules
1	3010	=C–H stretching	Lipid
2	2955	C–H assymetric stretching of –CH₃	Lipid
3	2925	Stretching of > CH₂ of acyl chains (assymetric)	Lipid
4	2850	Stretching of CH₂ of acyl chains (symetric)	Lipid
5	1745	C=O stretching	Lipid
6	1680–1640	Amide I band (C=O stretching)	Protein
7	1580–1520	Amide II (CONH bending)	Protein
8	1465	CH₂ deformtion	Lipid
9	1410	Amide III band (C–N stretching)	Protein
10	1380	CH₃ bending	Lipid
11	1240–1265	P=O stretching (assymetric) of > PO₂ phosphodiesters	Polyphosphate, phospholipid
12	1155	C–O–C stretching	Lipid
13	1080	P O stretching (symetric) of > PO₂	Polyphosphate, phospholipid
14	900–1200	C–O and C–C stretching, C–O–H and C–O–C deformation	Carbohydrate
15	875	P–O–P stretching	Polyphosphate, phospholipid
16	725	CH₂ deformation	Lipid

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