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Table 4 Activities of the Xyl-Doc α-l-ABFs on AXOS

From: The xyl-doc gene cluster of Ruminiclostridium cellulolyticum encodes GH43- and GH62-α-l-arabinofuranosidases with complementary modes of action

  A3Xa A2XXa XA3XXa A2+3XXa XA2+3XXa
GH4316-1229 NA NA NA NA NA
GH4310-1233 NA NA NA A3XX/A2XXb XA2XX/XA3XXb
GH62-1234 Araf/X2b
0.0510 ± 0.0028c
Araf/X3b
0.590 ± 0.043c
Araf/X4b
0.116 ± 0.005c
NA NA
GH62-CE6-1240 Araf/X2b
0.0300 ± 0.0005c
Araf/X3b
0.360 ± 0.028c
Araf/X4b
0.088 ± 0.0007c
NA NA
  1. NA not active
  2. aSubstrates listed according to the nomenclature established by Fauré et al. [32] are written as follow: X2: xylobiose, X3:xylotriose, X4: xylotetraose
  3. A3X: α-l-Araf-(1 → 3)-β-d-Xylp-(1 → 4)-d-Xylp
  4. A2XX: α-l-Araf-(1 → 2)-β-d-Xylp-(1 → 4)-β-d-Xylp-(1 → 4)-d-Xylp
  5. XA3XX: β-d-Xylp-(1 → 4)-[α-l-Araf-(1 → 3)]-β-d-Xylp-(1 → 4)-β-d-Xylp-(1 → 4)-d-Xylp
  6. A2+3XX: [α-l-Araf-(1 → 2)][α-l-Araf-(1 → 3)]-β-d-Xylp-(1 → 4)-β-d-Xylp-(1 → 4)-d-Xylp
  7. XA2+3XX: β-d-Xylp-(1 → 4)-[α-l-Araf-(1 → 2)][α-l-Araf-(1 → 3)]-β-d-Xylp-(1 → 4)-β-d-Xylp-(1 → 4)-d-Xylp
  8. bProducts of hydrolysis analyzed by HPAEC-PAD. The data show the mean and standard deviation of six independent experiments
  9. cSpecific activity in IU/µmol