Table 2 Primers used in this study
Primers | Primer sequence 5′ → 3′ |
|---|---|
pAWP89-ptac-For | TAGTTGTCGGGAAGATGCGT |
pAWP89-ptac-Rev | AGCTGTTTCCTGTGTGAATA |
FWD_speCEc | cacacaggaaacagctATGAAATCAATGAATATTGCC |
REV_speCEc | gtgaatacctTTACTTCAACACATAACCGT |
FWD_potE | tgttgaagtaaAGGTATTCACACAGGAAACAGCTATGAGTCAGGCTAAATCGA |
REV_potE | gcatcttcccgacaactaTTAACCGTGTTTATTTTTCAGT |
FWD_speCOb | ttcacacaggaaacagctATGACCGATCGTATCCGCGAA |
REV_speCOb | gcatcttcccgacaacta TCAGATCACGAAGGATTCGAG |
FWD_speE F1 | tggtctgacagttaccaGCCGGAAATGATGAAGTCCA |
REV_speE F1 | gggatttctTGCGCATTAGTTATCGAGGAG |
FWD_speE F2 | taatgcgcaAGAAATCCCTGCAGTGTTGA |
REV_speE F2 | ccgacaacctgcacatTTCGTTTAAGGTCTGTGCGA |
FWD_433speEc | TGTTGCCTTGTGCCGACAT |
REV_433speEc | ACCAACACCGGTAACGATAG |
FWD_argD | aggtattcacacaggaaacagctATGACCGGCCACATTATGC |
REV_argD | tgaatacctCTAGGGTTCATTGGCTTGATG |
FWD_argJ | tgaaccctagAGGTATTCACACAGGAAACAGCTATGGCGGTGGGGCAGGT |
REV_argJ | gcatcttcccgacaactaCTAAGTCCGATACTCCGCAT |
FWD_pTac-argDJ | atgtgcttcTCACATGTTCTTTCCTGCGT |
REV_pTac-argDJ | atgctatacgaagttatgcTCGAACTTTTGCTGAGTGGA |
FWD_351argDJ_F1 | acctgacgtctagatctgCGGCTACAACGTTCTGAAAG |
REV_351argDJ_F1 | gaagcacatGAAGCACATTCGAGCCTGAA |
FWD_351argDJ_F2 | gtgttaaccggtgagctTGTTTCAGGATGTCGCTTGG |
REV_351argDJ_F2 | ggatcctctagtgagctATGTTTAAAAGCTTCGCGGC |
FWD_351c | CATGACTTCATGAACGATGA |
REV_351c | TCCGACCAATAATCGTAATG |