Fig. 1

Effect of limiting nitrogen on the synthesis and accumulation of lipids in strain LS3. LS3 was cultivated for 72 h in YNB-based medium with carbon source at 30 g/L. The concentrations of 5 g/L and 0.75 g/L NH₄Cl led to a C/N ratio of 90 and 60, respectively, for cultures in glucose (G) and to a C/N ratio of 16 and 106, respectively, for cultures in a mixture of oleic acid and glucose (AO + G). a Cell density is expressed as OD₆₀₀ (white bars) and total lipid content as the percentage of dry-cell weight (black bars). Average values and standard deviations (n = 2) are shown in the histograms. b Relative proportion (%) of main FAs in each FA profile. c BODIPY-stained lipid droplets in cells sampled from low-nitrogen cultures in glucose (left) or in the presence of oleic acid (right)