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Table 2 Selected genes of lipid metabolism in the genome of B. raffinosifermentans LS3

From: Blastobotrys adeninivorans and B. raffinosifermentans, two sibling yeast species which accumulate lipids at elevated temperatures and from diverse sugars

Gene identifier Previous function/similarity [reference yeast]a New function prediction Amino acid % identity (coverage) Process
ARAD1B07414 Succinyl CoA ligase (alpha subunit) [sc] ATP:citrate lyase (subunit)
EC:2.3.3.8
88% (99%) with YALI0E34793 Formation of cytosolic acetyl-CoA, precursor of FA synthesis
ARAD1D32164 Succinyl CoA ligase (beta subunit) [sc] ATP:citrate lyase (subunit)
EC:2.3.3.8
82% (100%) with YALI0D24431 Formation of cytosolic acetyl-CoA, precursor of FA synthesis
ARAD1C08250 Acyl-CoA:diacylglycerol acyltransferase (DGAT) [sc] DGAT (no change)
EC:2.3.1.20
69% (59%) with YALI0E32769 Formation of TG
ARAD1D42460 Acyl-CoA:sterol acyltransferase [sc] DGAT
EC:2.3.1.20
42% (86%) with YALI0D07986 Formation of TG
ARAD1C30118 Weakly similar to FAT2 peroxisomal AMP binding protein [sc] Acyl-CoA synthetase 38% (99%) with YALI0E12419 (10 paralogs). PTS1 motifb Formation of acyl-CoA (peroxisomal activation of FA)
  1. aIn the current (first) version of the annotated genome of LS3 in GRYC database. First-round annotation was performed using BLAST against S. cerevisiae (sc) as the reference yeast to look for similarity
  2. bAKL in C terminus of the protein which likely targets it to peroxisome