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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Multifunctional elastin-like polypeptide renders β-glucosidase enzyme phase transition and high stability

Fig. 2

SDS–PAGE analysis of BglucLEH production and purification by Ni-NTA resin and ITC. Lane M: protein molecular weight marker (Broad); lane 1: crude cell lysate of cell with empty pET-28a(+) vector; lane 2: insoluble part of crude lysates containing BglucH; lane 3: crude cell lysate containing BglucH; lane 4: cell lysates of cell with BglucLEH plasmid without IPTG induction; lane 5: crude lysates of cells with BglucLEH expression; lane 6: insoluble part of crude lysates of cells with BglucLEH expression; lane 7: crude cell lysate containing BglucLEH; lanes 8, 9, 10: eluted BglucLEH with 20, 100, 400 mM imidazole, respectively; lanes 11, 12, 13, 14: BglucLEH purified by ITC with 1 M NaH2PO4, 1 M (NH4)2SO4, 1 M Na2SO4 or 2.5 M NaCl, respectively

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