Fig. 2
![Fig. 2](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs13068-019-1497-5/MediaObjects/13068_2019_1497_Fig2_HTML.png)
SDS–PAGE analysis of BglucLEH production and purification by Ni-NTA resin and ITC. Lane M: protein molecular weight marker (Broad); lane 1: crude cell lysate of cell with empty pET-28a(+) vector; lane 2: insoluble part of crude lysates containing BglucH; lane 3: crude cell lysate containing BglucH; lane 4: cell lysates of cell with BglucLEH plasmid without IPTG induction; lane 5: crude lysates of cells with BglucLEH expression; lane 6: insoluble part of crude lysates of cells with BglucLEH expression; lane 7: crude cell lysate containing BglucLEH; lanes 8, 9, 10: eluted BglucLEH with 20, 100, 400 mM imidazole, respectively; lanes 11, 12, 13, 14: BglucLEH purified by ITC with 1 M NaH2PO4, 1 M (NH4)2SO4, 1 M Na2SO4 or 2.5 M NaCl, respectively