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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Multifunctional elastin-like polypeptide renders β-glucosidase enzyme phase transition and high stability

Fig. 3

SDS–PAGE of fusion proteins BglucLEH purified by ITC method (a). Lane M: protein molecular weight marker (Broad); lane 1: crude lysate of cells with empty pET-28a(+) vector; lane 2: crude lysate of cells with BglucH expression; lane 3: crude lysate of cell with BglucLEH expression; lanes 4–7: purified BglucLEH by ITC using various (NH4)2SO4 concentrations (0.3, 0.5, 0.7, 1.0 M); lane 8: little proteins purified from crude lysate containing BglucH by ITC using 0.5 M of (NH4)2SO4; lane 9: BglucLEH purified by Ni-NTA resin. Purification efficiency by ITC method using various (NH4)2SO4 concentration (b)

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