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Table 1 Lignin composition of poplar transgenic milled wood particles and products of catalytic delignification

From: Overcoming cellulose recalcitrance in woody biomass for the lignin-first biorefinery

Genotype Lignin compositiona
H Gb OH-Gb S
DFRC (mol%) NMR (mol%) ESI (mol%) DFRC (mol%) NMR (mol%) ESI (mol%) DFRC (mol%) NMR (mol%) ESI (mol%) DFRC (mol%) NMR (mol%) ESI (mol%)
Control (INRA 717) (1) 0.7 ± 0.0 nd 1.7 ± 0.0 32.7 ± 0.0 29 25.2 ± 1.5 8.2 ± 0.2 66.6 ± 0.0 71 65.0 ± 1.3
AtC4H:F5H-37 (2) 0.6 ± 0.1 nd 0.8 ± 0.0 6.3 ± 0.4 6 19.2 ± 1.5 6.0 ± 0.2 93.1 ± 0.3 94 73.9 ± 1.4
AtC4H:F5H-64 (3) 0.6 ± 0.1 nd 1.0 ± 0.0 13.7 ± 0.3 13 21.2 ± 0.7 6.1 ± 0.2 85.7 ± 0.4 87 71.6 ± 0.5
AtC4H:F5H2 RNAi (4) 0.3 ± 0.5 nd 2.1 ± 0.1 47.2 ± 0.6 42 33.2 ± 1.0 11.2 ± 0.5 52.4 ± 1.0 58 53.5 ± 1.4
35S:F5H2 RNAi (5) 0.9 ± 0.2 nd 2.4 ± 0.1 42.2 ± 1.0 36 28.6 ± 0.3 12.2 ± 0.2 56.8 ± 12.0 64 56.8 ± 0.4
AtC4H:COMTa RNAi (6) 0.8 ± 0.0 nd 1.7 ± 0.1 44.8 ± 0.3 37 24.0 ± 1.1 32.2 ± 1.1 54.4 ± 0.3 63 42.0 ± 2.3
35S:COMTa RNAi (7) 0.0 ± 0.0 nd 1.6 ± 0.1 79.2 ± 0.6 64 39.1 ± 1.8 41.0 ± 3.5 20.8 ± 0.6 36 18.3 ± 1.7
  1. Composition in wood particles was determined by derivatization followed by reductive cleavage (DFRC); composition of reduced lignin units from catalytic conversion was determined by 2-D, 1H, 13C heteronuclear single quantum coherence (HSQC) NMR, and by electrospray ionization MS. Values from DFRC and ESI are the mean ± SD of three samples
  2. nd, not detected; –, not determined
  3. aDFRC was performed on milled wood particles; NMR and ESI were performed on the soluble liquors from catalytic delignification and reduction
  4. bNeither DFRC nor NMR can distinguish G and OH-G units; total G represents the sum of these units