Skip to main content

Table 1 Lignin composition of poplar transgenic milled wood particles and products of catalytic delignification

From: Overcoming cellulose recalcitrance in woody biomass for the lignin-first biorefinery

Genotype

Lignin compositiona

H

Gb

OH-Gb

S

DFRC (mol%)

NMR (mol%)

ESI (mol%)

DFRC (mol%)

NMR (mol%)

ESI (mol%)

DFRC (mol%)

NMR (mol%)

ESI (mol%)

DFRC (mol%)

NMR (mol%)

ESI (mol%)

Control (INRA 717) (1)

0.7 ± 0.0

nd

1.7 ± 0.0

32.7 ± 0.0

29

25.2 ± 1.5

–

–

8.2 ± 0.2

66.6 ± 0.0

71

65.0 ± 1.3

AtC4H:F5H-37 (2)

0.6 ± 0.1

nd

0.8 ± 0.0

6.3 ± 0.4

6

19.2 ± 1.5

–

–

6.0 ± 0.2

93.1 ± 0.3

94

73.9 ± 1.4

AtC4H:F5H-64 (3)

0.6 ± 0.1

nd

1.0 ± 0.0

13.7 ± 0.3

13

21.2 ± 0.7

–

–

6.1 ± 0.2

85.7 ± 0.4

87

71.6 ± 0.5

AtC4H:F5H2 RNAi (4)

0.3 ± 0.5

nd

2.1 ± 0.1

47.2 ± 0.6

42

33.2 ± 1.0

–

–

11.2 ± 0.5

52.4 ± 1.0

58

53.5 ± 1.4

35S:F5H2 RNAi (5)

0.9 ± 0.2

nd

2.4 ± 0.1

42.2 ± 1.0

36

28.6 ± 0.3

–

–

12.2 ± 0.2

56.8 ± 12.0

64

56.8 ± 0.4

AtC4H:COMTa RNAi (6)

0.8 ± 0.0

nd

1.7 ± 0.1

44.8 ± 0.3

37

24.0 ± 1.1

–

–

32.2 ± 1.1

54.4 ± 0.3

63

42.0 ± 2.3

35S:COMTa RNAi (7)

0.0 ± 0.0

nd

1.6 ± 0.1

79.2 ± 0.6

64

39.1 ± 1.8

–

–

41.0 ± 3.5

20.8 ± 0.6

36

18.3 ± 1.7

  1. Composition in wood particles was determined by derivatization followed by reductive cleavage (DFRC); composition of reduced lignin units from catalytic conversion was determined by 2-D, 1H, 13C heteronuclear single quantum coherence (HSQC) NMR, and by electrospray ionization MS. Values from DFRC and ESI are the mean ± SD of three samples
  2. nd, not detected; –, not determined
  3. aDFRC was performed on milled wood particles; NMR and ESI were performed on the soluble liquors from catalytic delignification and reduction
  4. bNeither DFRC nor NMR can distinguish G and OH-G units; total G represents the sum of these units