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Table 1 Growth kinetic parameters of engineered E. coli toward different concentrations of butanol

From: Enhancing butanol tolerance of Escherichia coli reveals hydrophobic interaction of multi-tasking chaperone SecB

Strain

0.8% Butanol

1.0% Butanol

1.2% Butanol

μa/h−1

Tb/%

μ/h−1

T/%

μ/h−1

T/%

Control

0.153 ± 0.009

16.2 ± 0.2

0.120 ± 0.007

10.1 ± 0.6

0.019 ± 0.009

2.86 ± 0.40

SecB

0.249 ± 0.027

27.5 ± 0.2

0.198 ± 0.018

20.0 ± 0.8

0.136 ± 0.016

9.13 ± 0.21

ClpB

0.196 ± 0.021

24.0 ± 1.2

0.139 ± 0.012

15.5 ± 1.2

0.074 ± 0.006

6.65 ± 0.22

YcdY

0.220 ± 0.014

21.5 ± 2.4

0.169 ± 0.021

12.3 ± 0.6

0.062 ± 0.005

6.53 ± 0.21

  1. aμ denotes specific growth rate between 0 and 2 h (Eq. 1)
  2. bT denotes tolerance to butanol (Eq. 2); All experiments were performed in triplicate. Growth curves of all the tested strains are available in Additional file
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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