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Table 1 Growth kinetic parameters of engineered E. coli toward different concentrations of butanol

From: Enhancing butanol tolerance of Escherichia coli reveals hydrophobic interaction of multi-tasking chaperone SecB

Strain 0.8% Butanol 1.0% Butanol 1.2% Butanol
μa/h−1 Tb/% μ/h−1 T/% μ/h−1 T/%
Control 0.153 ± 0.009 16.2 ± 0.2 0.120 ± 0.007 10.1 ± 0.6 0.019 ± 0.009 2.86 ± 0.40
SecB 0.249 ± 0.027 27.5 ± 0.2 0.198 ± 0.018 20.0 ± 0.8 0.136 ± 0.016 9.13 ± 0.21
ClpB 0.196 ± 0.021 24.0 ± 1.2 0.139 ± 0.012 15.5 ± 1.2 0.074 ± 0.006 6.65 ± 0.22
YcdY 0.220 ± 0.014 21.5 ± 2.4 0.169 ± 0.021 12.3 ± 0.6 0.062 ± 0.005 6.53 ± 0.21
  1. aμ denotes specific growth rate between 0 and 2 h (Eq. 1)
  2. bT denotes tolerance to butanol (Eq. 2); All experiments were performed in triplicate. Growth curves of all the tested strains are available in Additional file
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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